DESCRIPTION

• Mw: 28.9 kDa
• Tag: Myc-DDK
• Accn: NM_001003927
• Type: Human Tagged ORF Clone
• Refseq: NM_001003927.3
• Status: 2 Weeks*
• Symbol: EVI2A
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 2123
• Orf Size: 777 bp
• Synonyms: EVDA; EVI-2A; EVI2
• Refseq Orf: 780 bp
• Uniprot Id: P22794
• Refseq Size: 3010 bp
• Availability: 2 Weeks
• Cytogenetics: 17q11.2
• Gene Summary: May complex with itself or/and other proteins within the membrane, to function as part of a cell-surface receptor.[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC222392 representing NM_001003927 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGTTGCTTAGGAGTTGGTTTGGAAACAAAGACTTTCAGGCCCTACCTATACTAGCTAGATTGCCAAGCA TGCCCACGGACATGGAACACACAGGACATTACCTACATCTTGCCTTTCTGATGACAACAGTTTTTTCTTT GTCTCCTGGAACAAAAGCAAACTATACCCGTCTGTGGGCTAACAGTACTTCTTCCTGGGATTCAGTTATT CAAAACAAGACAGGCAGAAACCAAAATGAAAACATTAACACAAACCCTATAACTCCTGAAGTAGATTATA AAGGTAATTCTACAAACATGCCTGAAACATCTCACATCGTAGCTTTAACTTCTAAATCTGAACAGGAGCT TTATATACCTTCTGTCGTCAGCAACAGTCCTTCAACAGTACAGAGCATTGAAAACACAAGCAAAAGTCAT GGTGAAATTTTCAAAAAGGATGTCTGTGCGGAAAACAACAACAACATGGCTATGCTAATTTGCTTAATTA TAATTGCAGTGCTTTTTCTTATCTGTACCTTTCTATTTCTATCAACTGTGGTTTTGGCAAACAAAGTCTC TTCTCTCAGACGATCAAAACAAGTAGGCAAGCGTCAGCCTAGAAGCAATGGCGATTTTCTGGCAAGCGGT CTATGGCCCGCTGAATCAGACACTTGGAAAAGAACAAAACAGCTCACAGGACCCAACCTAGTGATGCAAT CTACTGGAGTGCTCACAGCTACAAGGGAAAGAAAAGATGAAGAAGGAACTGAAAAACTTACTAACAAACA GATAGGT ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC222392 representing NM_001003927 Red =Cloning site Green =Tags(s) MLLRSWFGNKDFQALPILARLPSMPTDMEHTGHYLHLAFLMTTVFSLSPGTKANYTRLWANSTSSWDSVI QNKTGRNQNENINTNPITPEVDYKGNSTNMPETSHIVALTSKSEQELYIPSVVSNSPSTVQSIENTSKSH GEIFKKDVCAENNNNMAMLICLIIIAVLFLICTFLFLSTVVLANKVSSLRRSKQVGKRQPRSNGDFLASG LWPAESDTWKRTKQLTGPNLVMQSTGVLTATRERKDEEGTEKLTNKQIG TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Druggable Genome, Transmembrane
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin