Anti-c-Abl (Tyr-245), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: ABL1
Host: Rabbit
Rrid: AB_2242125
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: c-Abl (Tyr-245)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: BCR-Abl, p150, Abelson murine leukemia viral oncogene homolog 1
Clonality: Rabbit Polyclonal
Immunogen: Phospho-c-Abl (Tyr-245) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 245 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Abl (Tyr-245) peptide (without carrier). On SDS-PAGE immunoblots of K-562 treated with pervanadate, the antibody detects a 145 kDa* protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl. In addition, this antibody detects a 145 kDa band in Jurkat cells treated with pervanadate.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 145
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways
Uniprot Number Immunogen Species: P00519
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-245