Anti-CXCR4 (C-terminal region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CXCR4
Host: Rabbit
Rrid: AB_10836952
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: CXCR4 (C-terminal region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; CXCR4
Clonality: Rabbit Polyclonal
Immunogen: CXCR4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human CXCR4. This sequence is conserved in mouse, rat, and chicken CXCR4, but has low homology to other CXCR family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects bands at 35, 42, 50, and 100 kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells. These bands co-migrate with similar bands detected with anti-CXCR4 (Ser-324/Ser-325) and anti-CXCR4 (a.a. 328-338) antibodies.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 35,42,50,100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.
Uniprot Number Immunogen Species: P61073