DESCRIPTION

• Aa: full length
• Mw: 120 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: U33284
• Species: Human
• Uniprot: Q14289
• Shiptemp: -80°C (dry ice)
• Synonyms: CADTK, CAK beta, CAK-beta, CAKB, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, PKB, Proline-rich tyrosine kinase 2, protein kinase B, protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK, Re
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Cytoplasmic Tyrosine
• References: 1. Cain R.J. et al., J Cell Biol. 2010 Mar 22,188(6):863-76. 2. Collins M. et al., Mol Immunol. 2010 May,47(9):1665-74.
• Description: Human PYK2 (GenBank Accession No. U33284), full length with N-terminal His-tag, MW=120 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: ADP-Glo assay with 0.1 mg/ml poly-(Glu4:Tyr) as a substrate and 100 mM ATP at final concentration. Reaction was done for 60 minutes at 30°C. ADP-Glo reagents were added for 45 minutes at RT, and Kinase detection reagents for 55 minutes at RT, followed by luminescence detection.
• Related Products: 78487
• Specific Activity: 5.6 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Cytoplasmic Tyr