DESCRIPTION

• Tag: Tag Free
• Accn: BC064014
• Type: Mouse Untagged Clone
• Refseq: BC064014, AAH64014
• Status: In Stock*
• Symbol: Mbtd1
• Vector: PCMV6-Kan/Neo
• Category: cDNA Clones
• Locus Id: 103537
• Synonyms: AA408199; AI194990; hemp
• Refseq Orf: 1869 bp
• Insert Size: 1869 bp
• Refseq Size: 3785 bp
• Availability: In Stock
• Cytogenetics: 11 D
• Gene Summary: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Specifically binds to monomethylated and dimethylated 'Lys-20' on histone H4 (By similarity).[UniProtKB/Swiss-Prot Function]
• Sequence Data: Fully Sequenced ORF ( show hide ) >BC064014 CCCGAGTTAAAAGGGGGGAAATCCGGGGGCTGCCACTGCTGTCGCTGGCCCTGGCCCAGCCGGGGGTCCC CCCTGTAGTCGCCGCAGTCCCGGGGAGAGGCACCGGCCCCCTGCTCGCGGGGGGGGAGGGGGCGCCGCGG GCTCGGGGAGCACGGACGGCCCCTACCCATGAGGCCCTGATGGAAAACACAAAGGATCTGAGTCGGAAGA GACACTAGAAGTCACCCAAAGCAGCCCAGCTTCTGACGAGTGGGTTTCTGTAAGCCTCCACTCAGAGATG TGATGATCACTGCAATTAAAAAACAGAACATTCTTCACAATCCAAAAGAAAAAGCAGACTGGTTCGGGAT GTTTGACGGCTATGATAGCTGCAGTGAGGACACAAGTAGCAGCTCCAGCTCTGAGGAGAGTGAAGAAGAA GTTGCTCCTTTACCTTCCAATCTCCCAATCATCAAGAATAATGGACAAGTCTACACATACCCAGATGGTA AATCTGGCATGGCTACCTGTGAGATGTGTGGGATGGTCGGTGTGCGAGATGCTTTTTACTCTAAAACGAA ACGTTTCTGCAGTGTTTCCTGTTCAAGAAGTTACTCGTCAAACTCTAAGAAGGCAAGCATTCTGGCCAGA CTTCAGGGTAAGCCTCCAACAAAGAAAGCAAAAGTCCTTCAAAAACAACCTTTAGTTGCTAAATTAGCTG CCTATGCCCAGTATCAAGCTACCTTGCAAAATCAAGCAAAGACAAAAGCAGGCAATTCTGCAATCTCTGT GGAAGGCTTCAGCTGGGGTAACTACATCAACAGCAACAGCTTCATAGCAGCTCCAGTGGCCTGTTTTAAG CATGCACCTATGGGGACCTGCTGGGGTGATATCTCGGAAAATGTAAGGATAGAAGTTCCCAATACAGACT GCAGTCTACCTACCAAAGTCTTCTGGATTGCTGGAATTATAAAATTAGCAGGTTATAATGCCCTTTTGAG ATATGAAGGATTTGAAAATGATTCTTCTCTGGACTTCTGGTGCAATATATGTGGGTCTGATATTCATCCA GTTGGTTGGTGTGCAGCTAGTGGAAAACCTCTCGTTCCTCCTAGAACTGTTCAACATAAATATACAAACT GGAAAGCTTTTCTAGTAAAAAGACTTACTGGTGCCAAAACACTTCCTCCTGATTTCTCACAAAAGGTTTC TGAGAGTATGCAATATCCTTTCAAACCTTGCATGAGAGTAGAAGTAGTTGACAAGAGGCATTTATGTCGA ACAAGAGTAGCAGTGGTGGAAAGTGTAATTGGAGGACGACTACGGCTGGTGTATGAAGAGAGTGAAGATA GAACAGACGACTTCTGGTGCCACATGCACAGCCCCTTAATCCACCATATTGGATGGTCAAGAAGCATAGG CCATCGATTCAAGAGATCAGATATTACGAAGAAACAGGACGGACATTTCGATACACCTCCACACTTATTT GCTAAGGTAAAAGAAGTAGACCAGAGTGGAGAATGGTTCAAAGAAGGAATGAAATTGGAAGCTATAGACC CATTAAATCTTTCTACAATATGTGTTGCCACCATTAGAAAGGTGCTGGCTGATGGATTCCTGATGATTGG GATTGATGGCTCAGAAGCAGCAGATGGATCTGACTGGTTCTGTTATCATGCAACCTCTCCTTCCATTTTC CCTGTGGGTTTCTGTGAAATTAACATGATAGAACTGACTCCACCCAGAGGTTACACAAAACTTCCTTTTA AATGGTTTGACTACCTCAGGGAAACCGGCTCCATTGCAGCACCAGTAAAACTATTTAATAAGGATGTTCC AAACCACGGATTCCGTGTAGGAATGAAATTAGAAGCTGTAGATCTCATGGAGCCACGGTTAATATGTGTA GCCACAGTTACTCGAATTATTCACCGTCTCTTGAGGATACATTTTGATGGTTGGGAAGAAGAGTATGACC AGTGGGTAGACTGTGAGTCCCCTGACCTCTATCCTGTAGGGTGGTGTCAGTTAACTGGATATCAACTACA GCCTCCAGCATCACAGTCATCAAGAGAAAGCCAATCAGCTTCTTCAAAACAGAAGAAAAAGGCTAAGTCT CAGCAATACAAAGGACATAAGAAAAGTACTGAAGAAATGACGGAGTCTTAGCTGTAAAGTTGATAGACCA TGAAGATGAACCCCAGTGCGTTCCAGAAGACTTGGTAACAGCCGTGCGTTTCTGTCTGTCTCATACAGTG ACCACGTCACAGCTGAAGGAGGAGCTGCTGGACGGGGAGGACTATAGCTTCCTCCATGGAGCATCTGACC AGGAAAGCAATGGCTCTGCCACCGTCTACATCAAACAAGAGCCATGAGGCGACTCGGAAACCATGGGCAG GCGGGGCTGTTTACAGGACTGATTTGGAATCAGCCAGCTGTATAGCGGGCTATTCTACTGGGACATTTTG CTAAACACAGAAAAAAGTTCAGTTCCAGATTTTTCAGGTGGGGGGGAAACTATTTTGGTGGGGGGGCAAT TTTTCAATTTATAAAGACGGACAATTTTTGTGTTGTATTTGAAGCTTTTGAAAGAATTTTGTAATATTTT CCAAGTTTGGATTTATGTGCATTGTTAACAAGAACTGAAATTATAACTTTTTTGGTAAGATAAAAGTTTA GGTAGCAGGATTGAAGGAAATGATTAAGAAGGATATAGTTGTAAATGCAAATGAACTGTCATTACAAATG AACCTTCTTGGTACCTGTTGGGAGATTTTTTGGATTTTAGAGTTAGGCCAGTCACATCTCCAGCTTCCTT TGCTGCGAAAATATGCAACTGAACCCCTCACAGAGGGCTCATCACCATGTAGATCACGGAAGGGGTCATT AATTGTGCTCGCTGACGTTTTATTGCAGCCCATTTAACTGTTTGTACAGAAACTTTTTCATTCTGCTAAA ATTTATTTGGAGTTGTATATGAAACTAGGAGAACTCTGGATACTTTTATATGCTCTCCTTCACGTAAAGA TGATTAAAATTGTCTAACACTAAAGTGTTAAACTGGAATGGTTGACAAAGTACACAAGATCTCAGTCTAC ATAAAGGGTTGGGGGAAATTAGTATTTTCCTAAGTTTATTCTGTTTTCCTTGCTAGAAAAATCCCATAAA AGGGATATTCTTGTCAGACCTCTGCCATTTCTTCCATCTGTGAGAGAGCAACGTGATGTAACCACACCTG AGAGCAGGTACTGTCCTGTGTTACAAAAGAATCACATGGAAAATTGTTAACCAAAATAAGTGTAGATTTC AGAATCATTCCAGCTCTACTCTTAACTGTCCTGAATTTGTTAGAAACTGATTTGAAAGAAATGTTTCTTA AAATTCCATACACACACACACACATATTATGAATTGTCTTTAATTGTAGTCAGAATTTATTATAACAATA TTTGATTTGGACCATTTTAAACATTCCCTATTTTAAAATTCATACGGCTTCCTTAAGAAGTAGAATGAAA GGGTAAATTGGTGACATGTTTGCTCTCTGCATTTTCTAACCTTCTACCGAATTGTGACTGACTCAGAGAG CTCTAGCATTTACCAGTGAGGTTCAGAAACTAAACTCTCAGGAATTCATTGCATCTGTTGTAGAAGTGCT TCTGGGTCTGAGCCTGGCCTCCTCAGAGTGGTAATACTGCCCACTTCCTCTGGAAAATAGGCAGGGCTAA TGAGAAACTAATCAAATGATTAACCTCTGCGGCCCTTCAGCCTTTGGAATGCTTTTAAAAAAAAAAAAAA AAAAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: RsrII-NotI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin