78584
IL-2: IL-2RA Inhibitor Screening Colorimetric Assay Kit
BPS Bioscience
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DESCRIPTION
The IL-2: IL-2 Receptor Alpha (IL-2RA) Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of inhibitors or neutralizing antibodies blocking the interaction between human IL-2 and its receptor IL-2RA.
The assay requires only a few steps. First, IL-2RA is coated on a 96-well plate overnight. After blocking, the receptor is pre-incubated with the test inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-IL-2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.
DETAILS
- Notes: Troubleshooting Guide: Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
- Uniprot: P01589
- Shiptemp: -80°C (dry ice)
- Warnings: Avoid freeze/thaw cycles
- Category: Cytokine/Assay Kit
- Background: Interleukin-2 (IL-2) is a cytokine secreted by activated T lymphocytes to stimulate the proliferation of both B and T cells. IL-2 is involved in the extreme inflammation response termed "cytokine storm" observed following viral infection in susceptible patients or following adaptive cell therapy. Thus, it is considered a therapeutic target for the treatment of severe COVID-19. IL-2 acts by binding to a membrane heterotrimeric receptor consisting of IL-2 receptor alpha (IL2RA, also known as CD25) and beta (IL2RB) chains, which together with the common gamma chain (IL2RG) form a high-affinity receptor, whereas IL-2RA homodimers form a low-affinity receptor. Polymorphisms in the genes coding for IL2RA and IL2RB may be associated with risk of lung cancer.
- Description: The IL-2: IL-2 Receptor Alpha (IL-2RA) Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of inhibitors or neutralizing antibodies blocking the interaction between human IL-2 and its receptor IL-2RA. The assay requires only a few steps. First, IL-2RA is coated on a 96-well plate overnight. After blocking, the receptor is pre-incubated with the test inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-IL-2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.
- Methodology: Colorimetric
- Supplied As: The kit is provided in a convenient 96-well format, with Biotinylated-IL-2, purified IL-2RA protein, Streptavidin-HRP, and assay buffers for 100 reactions.
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Screen inhibitors of IL-2 binding to IL-2RA.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 60481, 101203, 101381, 101593
- Contraindications: DMSO concentration in the final reaction should be ≤1%.
- Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
- Scientific Category: Cytokine