DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Cell Line
• References: Abe, M. et al. (1994) An assay for transforming growth factor- β using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. Analytical Biochemistry 216:276-584. Khan, S. et al. (2012) Quantification of active and total transforming growth factor-β levels in serum and solid organ tissues by bioassay. BMC Research Notes 5: 636
• Description: PAI-1 Reporter (Luc)-Mv1 Lu cell line is designed for monitoring transforming growth factor β (TGF-β)-induced plasminogen activator inhibitor-1 (PAI-1) expression. Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression. PAI-1 Reporter (Luc) -Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity.
• Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO.
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: Monitor TGF-β signaling pathway activity.
• Host Species: Aleutian mink
• Product Type: Cell Line
• Host Cell Line: Mv1 Lu
• Biosafety Level: BSL-1
• Related Products: 90901-1, 90900-1, 60653, 40707, 60690, 60187, 79796
• Storage Stability: Store in liquid nitrogen immediately upon receipt.
• Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
• Scientific Category: Immunotherapy
• Instructions for Use: See data sheet for detailed culturing and assay protocol.
• Required Accessories: 60690,60187,79796
• Signed License Required: Yes