DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Genbank: NM004460.2
• Species: Human
• Shiptemp: -80°C (dry ice)
• Synonyms: FAP-alpha cell line, Fibroblast activation protein alpha cell line, prolyl endopeptidase FAP cells, 79947-H, 79947-M, 79947-L
• Warnings: Avoid freeze/thaw cycles.
• Category: Cancer Therapy Target
• Background: Fibroblast activation protein alpha (FAP-alpha) belongs to the dipeptidyl peptidase IV (DPP4; CD26) gene family and is a cell-surface serine protease that acts on various hormones and extracellular matrix components. FAP expression under physiological conditions is scarce in healthy adult tissues. But FAP gene is highly upregulated in a wide variety of cancers, and is often used as a marker for pro-tumorigenic stroma. It has also been proposed as a molecular target of cancer therapies, and a great deal of research has gone into design and testing of diverse FAP-targeted treatments.
• Description: Recombinant FAP-CHO K1 cell line stably expressing a full length human FAP-alpha protein (Genbank accession No: NM004460.2). Surface expression of FAP was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of FAP expression (High, Medium, or Low) to mimic different stages of cancer target cells with various FAP expression levels.
• Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: • Screen for activators or inhibitors of antibody-mediated signaling for immunotherapy research and drug discovery.• Characterize FAP antibodies and ligands in binding assays.
• Host Species: hamster
• Product Type: Cell Line
• Host Cell Line: CHO-K1
• Biosafety Level: BSL-1
• Related Products: 80210, 80100
• Storage Stability: Store in liquid nitrogen immediately upon receipt.
• Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
• Scientific Category: Protease
• Instructions for Use: See assay protocol for detailed instructions.
• Required Accessories: 60186,79529,79796
• Signed License Required: Yes