LMX1B (NM_001174146) Human Tagged Lenti ORF Clone

DESCRIPTION:

Lenti ORF clone of Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 3, mGFP tagged

DETAILS

Mw: 45.5 kDa
Tag: mGFP
Accn: NM_001174146
Type: Human Tagged ORF Clone
Refseq: NM_001174146.1
Status: In Stock*
Symbol: LMX1B
Vector: pLenti-C-mGFP
Category: cDNA Clones
Locus Id: 4010
Orf Size: 1218 bp
Synonyms: FSGS10; LMX1.2; NPS1
Refseq Orf: 1221 bp
Availability: In Stock
Cytogenetics: 9q33.3
Gene Summary: This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(RC230016).
Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
E Coli Selection: Chloramphenicol (34 ug/mL)
Protein Families: Transcription Factors
Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Mammalian Cell Selection: None