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10231-H08H

Human LDLR / LDL Receptor Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the extracellular domain of human LDLR (NP_000518.1) precursor (Met 1-Arg 788) was expressed with a C-terminal polyhistidine tag.

DETAILS

  • Tag: C-His
  • Purity: > 85 % as determined by SDS-PAGE
  • Source: HEK293 Cells
  • Species: Human
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Immobilized Human LDLR/LDL Receptor His (Cat:10231-H08H) at 2 μg/mL (100 μL/well) can bind Mouse PCSK9 mFc (Cat:50251-M05H), the EC50 of Mouse PCSK9 mFc (Cat:50251-M05H) is 100-800 ng/mL.
  • Molecule: LDLR
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4
  • Molecular Mass: The secreted recombinant human LDLR comprises 777 amino acids with a predicted molecular mass of 86 kDa. As a result of different glycosylation, it migarates with the apparent molecular mass of 110-140 kDa in SDS-PAGE under reducing conditions corresponding to the mature and immature form.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: NP_000518.1
  • Predicted N Terminal: Ala 22