71173-1 71173-2
CD160, His-tag (Human) HiP™ Recombinant
BPS Bioscience
DESCRIPTION
Human secreted CD160, also known as Natural killer cell receptor BY55, GenBank Accession No. NM_007053, a.a. 27-159 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 15.6 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
DETAILS
- Aa: 27-159
- Mw: 15.6 kDa
- Tags: C-Terminal His-tag
- Notes: HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.
- Format: Aqueous buffer solution.
- Genbank: NM_007053
- Species: Human
- Uniprot: O95971
- Shiptemp: -80°C (dry ice)
- Synonyms: Natural killer cell receptor BY55
- Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
- Category: Immunotherapy/Protein
- References: 1. Cai, G., et al., Nat. Immunol. 2008; 9: 176-185. 2. Le Bouteiller, P. et al., Proc. Nat. Acad. Sci. USA 2002; 99(26): 16963- 16968.
- Description: Human secreted CD160, also known as Natural killer cell receptor BY55, GenBank Accession No. NM_007053, a.a. 27-159 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 15.6 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
- Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for studying protein binding and screening small molecules.
- Host Species: human
- Product Type: Protein
- Host Cell Line: HEK293
- Biosafety Level: Not applicable (BSL-1)
- Endotoxin Level: <1 EU/µg
- Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Casein substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent (Promega).
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Immunotherapy
- Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.