DESCRIPTION

• Aa: 27-159
• Mw: 15.6 kDa
• Tags: C-Terminal His-tag
• Notes: HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.
• Format: Aqueous buffer solution.
• Genbank: NM_007053
• Species: Human
• Uniprot: O95971
• Shiptemp: -80°C (dry ice)
• Synonyms: Natural killer cell receptor BY55
• Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
• Category: Immunotherapy/Protein
• References: 1. Cai, G., et al., Nat. Immunol. 2008; 9: 176-185. 2. Le Bouteiller, P. et al., Proc. Nat. Acad. Sci. USA 2002; 99(26): 16963- 16968.
• Description: Human secreted CD160, also known as Natural killer cell receptor BY55, GenBank Accession No. NM_007053, a.a. 27-159 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 15.6 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
• Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for studying protein binding and screening small molecules.
• Host Species: human
• Product Type: Protein
• Host Cell Line: HEK293
• Biosafety Level: Not applicable (BSL-1)
• Endotoxin Level: <1 EU/µg
• Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Casein substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent (Promega).
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Immunotherapy
• Instructions for Use: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.