Anti-Spectrin Alpha 1 (Erythrocyte Marker) Monoclonal Antibody(Clone: SPTA1/2939R)

DESCRIPTION

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

DETAILS

Gene: SPTA1
App Id: ELISA,WB,IHC
Gene Id: 6708
Isotype: Rabbit IgG
Clone Name: SPTA1/2939R
Uniprot Id: P02549
Host Animal: Rabbit
Clonality Id: Monoclonal
Reactivity Id: Human
Product Content: 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Alternative Name: Alpha I spectrin; Elliptocytosis 2 (EL2); Erythrocyte alpha spectrin; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha erythrocytic 1; SPH3; SPTA1
Application Note: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Immunogen Information: Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)