50440-M08H
Mouse ICAM-1 / CD54 Protein (His Tag)
Sino Biological
DESCRIPTION
A DNA sequence encoding the extracellular domain of mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was fused with a polyhistidine tag at the C-terminus.
DETAILS
- Tag: C-His
- Purity: ≥ 96 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: Mouse
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 40% cells will adhere specifically.
- Molecule: ICAM1
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4
- Molecular Mass: The recombinant mouse ICAM1 consists of 469 amino acids after removal of the signal peptide. and has a predicted molecular mass of 51.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1 is approximately 80-90 kDa due to glycosylation.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_034623.1
- Predicted N Terminal: Gln 28