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40592-V08H9

SARS-CoV-2 (2019-nCoV) Spike RBD-His (W436R) Recombinant Protein

Sino Biological

DESCRIPTION

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(W436R)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(W436R)) was expressed with a polyhistidine tag at the C-terminus.

DETAILS

  • Tag: C-His
  • Purity: > 95 % as determined by SDS-PAGE.
  • Source: HEK293 Cells
  • Species: SARS-CoV-2
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by its binding ability in a functional ELISA. Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (W436R) Recombinant Protein (40592-V08H9), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (W436R) Recombinant Protein (40592-V08H9) is 20-50 ng/mL.
  • Molecule: Spike
  • Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4.
  • Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(W436R)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.51 kDa.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: YP_009724390.1
  • Predicted N Terminal: Arg319