DESCRIPTION

• Uniprot: Q9NR22
• Shiptemp: -80°C (dry ice)
• Synonyms: protein arginine N-methyltransferase 8, PRMT8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Assay Kit
• References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
• Description: The PRMT8 Chemiluminescent Assay Kit is designed to measure PRMT8 activity for screening and profiling applications. The key to the PRMT8 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: The PRMT8 Chemiluminescent Assay Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethio
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 52058, 51052
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Methyltransferase
• Instructions for Use: See assay kit datasheet for detailed protocol.