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80012-RNAE

Rat CNTF / Ciliary Neurotrophic Factor Protein

Sino Biological

DESCRIPTION

A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.

DETAILS

  • Tag: Native
  • Purity: > 92 % as determined by SDS-PAGE
  • Source: E. coli
  • Species: Rat
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: 1. Immobilized rat CNTF (Cat: 80012-RNAE) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: 80019-R08B) with a linear range of 1.56-50.0 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-100 ng/ml.
  • Molecule: CNTF
  • Endotoxin: Please contact us for more information.
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5
  • Molecular Mass: The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: P20294
  • Predicted N Terminal: Met 1