36-3365-20 36-3365
Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: VIM/3736)
Abeomics
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DESCRIPTION
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
DETAILS
- Gene: VIM
- App Id: WB,IHC
- Gene Id: 7431
- Isotype: Mouse IgG2b, kappa
- Clone Name: VIM/3736
- Uniprot Id: P08670
- Host Animal: Mouse
- Clonality Id: Monoclonal
- Reactivity Id: Human
- Product Content: 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
- Alternative Name: VIM
- Application Note: Western Blot (2-4ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
- Storage Condition: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
- Immunogen Information: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
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