DESCRIPTION

• Mw: 60.2 kDa
• Tag: Myc-DDK
• Accn: NM_001008295
• Type: Rat Tagged ORF Clone
• Refseq: NM_001008295.1, NP_001008296.1
• Status: 5 Weeks*
• Symbol: Fip1l1
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 289582
• Orf Size: 1608 bp
• Synonyms: MGC93674
• Refseq Orf: 1611 bp
• Uniprot Id: Q5U317
• Refseq Size: 1925 bp
• Availability: 5 Weeks
• Cytogenetics: 14p11
• Gene Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity).[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RR212765 representing NM_001008295 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGTCGGCCGGCGAGGTGGAGCGTCTGGTGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGT GGCTGTATGGCGGCCCGTGGGACGTGCATGTGCACAGTGATTTGGCAAAGGATCTAGATGAAAATGAAGT TGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCTTCTGGAATTGAAGAGGAAGCTGCTGAAAAT GGCGTGGCGAAACCGAAAGTGACCGAGACTGAAGACGACAGTGATAGTGACAGTGACGATGATGAAGATG ACGTTCACGTTACTATTGGAGACATCAAAACGGGAGCTCCACAGTATGGGAGTTATGGAACAGCACCAGT AAATCTTAATATCAAGGCAGGGGGAAGAGTTTATGGAAATACAGGAACCAAAGTCAAAGGAGTAGACCTC GATGCCCCTGGCAGCATTAATGGAGTTCCACTCTTGGAAGTAGATTTGGATTCTTTTGAAGATAAACCAT GGCGGAAACCTGGTGCTGATCTTTCCGATTATTTTAATTATGGCTTTAATGAAGATACTTGGAAAGCTTA CTGTGAAAAACAAAAGAGGATCCGAATGGGACTGGAAGTTATACCCGTTACTTCAACGACAAACAAGATT ACGGTACAGCAAGGGAGAACTGGAAATTCAGAGAAAGAAGCAGCACTTCCATCTACAAAAGCTGAGTTTA CTTCTCCGCCATCATTGTTCAAGACTGGTCTGCCACCAAGCAGGAGATTACCTGGAGCAATCGATGTCAT TGGTCAAACAATAACCATCAGCCGAGTAGAAGGAAGACGGCGTGCAAATGAGAACAGCAATATACAGGTT CTTTCTGACAGATCTGCCACTGAAGTAGACAACAATTTCAGCAAACCACCTCCATTTTTCCCTCCAGGGG CTCCTCCTACTCACCTTCCTCCTCCTCCATTTCTTCCACCTCCTCCAACTGTCAGCACTGCTCCACCTTT GATTCCACCACCAGGTTTCCCCCCTCCACCAGGCGCTCCACCTCCATCTCTTATACCGACAATAGAAAGT GGCCATTCCTCTGGTTACGACAGCCGTTCTGCACGGGCGTTTCCATATGGCAATGTTGCCTTTCCCCACC TTACCAGTTCCGCTCCCTCATGGCCTAGCCTTGTGGATACCACTAAGCAGTGGGACTATTATGCACGGAG AGAAAAAGATCGAGATCGAGACCGAGAGAGAGACCGAGACAGAGAAAGGGAGAGAGACCGGGACAGAGAA AGGGAGCGCACCCGGGAGCGAGAGCGGGAGCGGGACCACAGCCCCACCCCAAGTGTTTTCAACAGTGATG AAGAGCGATACAGATACAGGGAGTATGCAGAGAGAGGCTATGAGCGCCACAGAGCTAGTCGGGAGAAAGA AGAGCGGCACAGAGAACGACGGCACAGAGAGAAGGAGGAAACAAGACACAAGTCCTCTCGCAGTAATAGT AGACGTCGTCATGAAAGTGAAGAAGGGGACAGTCACAGAAGACACAAGCACAAAAAGTCTAAAAGGAGCA AAGAAGGAAAAGAGGCTGGCAGCGAGCCGGTCCCTGAGCAGGAGAGCACGGAAGCTGCCCCTGCCGAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RR212765 representing NM_001008295 Red =Cloning site Green =Tags(s) MSAGEVERLVELSGGTGGDEEEEWLYGGPWDVHVHSDLAKDLDENEVERPEEENASANPPSGIEEEAAEN GVAKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKAGGRVYGNTGTKVKGVDL DAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNKI TVQQGRTGNSEKEAALPSTKAEFTSPPSLFKTGLPPSRRLPGAIDVIGQTITISRVEGRRRANENSNIQV LSDRSATEVDNNFSKPPPFFPPGAPPTHLPPPPFLPPPPTVSTAPPLIPPPGFPPPPGAPPPSLIPTIES GHSSGYDSRSARAFPYGNVAFPHLTSSAPSWPSLVDTTKQWDYYARREKDRDRDRERDRDRERERDRDRE RERTRERERERDHSPTPSVFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNS RRRHESEEGDSHRRHKHKKSKRSKEGKEAGSEPVPEQESTEAAPAE TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin