Human Integrin alpha 10 beta 1 (ITGA10&ITGB1) Heterodimer Protein, His Tag&Tag Free

DESCRIPTION

Human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.

DETAILS

Tag: C-10×His | Native
Host: HEK293
Size: 100ug
Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
Format: Powder
Purity: 95%
Source: Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 23 - Ser 1122 (ITGA10) & Gln 21 - Asp 728 (ITGB1) (Accession # O75578-1 (ITGA10) & P05556-1 (ITGB1)).
Storage: -20℃
Category: Bio-Markers & CD Antigens
Molecule: Integrin alpha 10 beta 1
Accession: NP_003628.2 (ITGA10) & NP_002202.2 (ITGB1)
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Phe 23 - Ser 1122 (ITGA10) & Gln 21 - Asp 728 (ITGB1)
Swiss-prot: O75578-1 (ITGA10) & P05556-1 (ITGB1)
Package Size: 100ug*1
Characteristics: Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA10 and ITGB1, has a calculated MW of 126.6 kDa (ITGA10) and 83.7 kDa (ITGB1). Subunit ITGA10 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 140-160 kDa (ITGA10) and 100-130 kDa (ITGB1) respectively due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 126.6 kDa (ITGA10) & 83.7 kDa (ITGB1)
Shipping Condition: RT
Species Reactivity: Human
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