DESCRIPTION

• Category: REAGENTS
• Keywords: fvtsbanqpcr, SNP, Genotyping, HRM, High Resolution Melt, Gene Scanning, CpG Methylation, SNP Detection, qPCR, Real Time PCR, qpcr reagents, mutation scanning, cdna, dsDNA, polymorphisms, mutations, mutation, epigenetics, HRM analysis, high resolution melt analysis
• Paragraph 1: High Resolution Melt (HRM) analysis is a powerful post-PCR analysis method used to detect and identify mutations, polymorphisms and epigenetic differences in double-stranded DNA samples.
• Paragraph 2: qPCRBIO HRM Mix uses the novel SyGreen 2 intercalating dye. SyGreen 2 does not inhibit PCR and is a saturating, 3rd generation dye essential for accurate SNP genotyping. Unlike first generation dyes, SyGreen 2 can be used at high concentrations and does not have a different affinity for AT or GC rich sequences. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. qPCRBIO HRM Mix delivers accurate detection of subtle sequence variations including class I to class IV mutations and CpG methylation differences. Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO HRM Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.
• Bullet Point 1: Accurate and fast detection of genetic mutations and SNPs
• Bullet Point 2: Detects subtle sequence variations including class I to class IV mutations
• Bullet Point 3: Discriminates CpG methylation differences
• Bullet Point 4: Utilizes SyGreen 2, a 3rd generation dye that produces ultra-sensitive melt profiles
• Bullet Point 5: Efficient specific amplification from GC rich and AT rich sequences
• Bullet Point 6: Universal compatability on all qPCR machines capable of HRM in both fast and standard cycling conditions
• Manufacturer Sku: PB20.31-20