Anti-Mesothelin CAR Lentivirus (P4 ScFv-CD8-4-1BB-CD3ζ)

DESCRIPTION

The Anti-mesothelin CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain fragment variable) of anti-mesothelin (Clone P4) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1)._x000D_ A _x000D_ _x000D_ B_x000D_ _x000D_ Figure 1. (A) Schematic of the lenti-vector used to generate the anti-mesothelin CAR lentivirus and (B) Construct diagram showing components of the anti-mesothelin CAR.

DETAILS

Notes: Biosafety: The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions. License Disclosure: Visitbpsbioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
Uniprot: Q13421
Shiptemp: -80°C (dry ice)
Synonyms: CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, Megakaryocyte-potentiating factor (MPF), MSLN, MPF
Warnings: Avoid freeze/thaw cycles
Category: CAR-T/Lentivirus
Background: Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently overexpressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy, including drug-conjugated antibodies and chimeric antigen receptor T cells (CAR-T Cells).
Description: The Anti-mesothelin CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain fragment variable) of anti-mesothelin (Clone P4) linked to a 2nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1)._x000D_ A _x000D_ _x000D_ B_x000D_ _x000D_ Figure 1. (A) Schematic of the lenti-vector used to generate the anti-mesothelin CAR lentivirus and (B) Construct diagram showing components of the anti-mesothelin CAR.
Formulation: The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.
Supplied As: 50 µl of anti-mesothelin CAR at a titer ≥ 3x108 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: Positive control for anti-mesothelin CAR evaluation in T cells; useful for transduction optimization.
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 90184-A, 79777, 79059, 78132
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C.
Scientific Category: CAR-T