CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit

DESCRIPTION

The CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-1 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

DETAILS

Uniprot: P10747
Shiptemp: -80°C (dry ice)
Synonyms: CD80, B7.1
Warnings: Avoid freeze/thaw cycles.
Category: Immunotherapy/Assay Kit
Background: The activation of naive T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-1 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.
References: 1. Keir, M.E., et al. Immunol. Rev. 2005, 204: 128-143. 2. Haas, C., et al. Int. J. Cancer. 2006, 1;118: 658-667.
Description: The CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-1 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Methodology: Chemiluminescent
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CD28, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Unspsc Code: 41105332
Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
Applications: This kit is useful for screening for inhibitors of B7-1 binding to CD28.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Related Products: 72003, 79572, 79512
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Scientific Category: Cell Surface Receptor/Single-pass Transmembrane Protein
Instructions for Use: See assay kit datasheet for detailed protocol.