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RC222417L4

NFAT2 (NFATC1) (NM_172388) Human Tagged Lenti ORF Clone

Origene Technologies, Inc.

DESCRIPTION

Lenti ORF clone of Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1), transcript variant 4, mGFP tagged

DETAILS

  • Mw: 38.4 kDa
  • Tag: mGFP
  • Accn: NM_172388
  • Type: Human Tagged ORF Clone
  • Refseq: NM_172388.1
  • Status: 3 Weeks*
  • Symbol: NFAT2
  • Vector: pLenti-C-mGFP-P2A-Puro
  • Category: cDNA Clones
  • Locus Id: 4772
  • Orf Size: 1059 bp
  • Synonyms: NF-ATC; NF-ATc1.2; NFAT2; NFATc
  • Refseq Orf: 1062 bp
  • Uniprot Id: O95644
  • Refseq Size: 3671 bp
  • Availability: 3 Weeks
  • Cytogenetics: 18q23
  • Gene Summary: The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]
  • Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(RC222417).
  • Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
  • Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
  • Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
  • E Coli Selection: Chloramphenicol (34 ug/mL)
  • Protein Families: Druggable Genome, Transcription Factors
  • Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
  • Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
  • Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
  • Mammalian Cell Selection: Puromycin