11846-HNAE
Human IL4 / Interleukin-4 Protein
Sino Biological
DESCRIPTION
A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) was expressed and purified with an initial Met.
DETAILS
- Tag: Native
- Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
- Source: E. coli
- Species: Human
- Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.25 ng/mL. 2.Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with IL4 (Cat# 11846-HNAE) (100 ng/mL, 15min; +), using Phospho-Stat6 (Tyr641) Antibody, Rabbit MAb at 1:10000 dilution (upper) or Anti-STAT6 Antibody, Rabbit PAb (Cat# 201061-T36) at 1:1000 dilution (lower) (Routinely tested).
- Category: Proteins
- Molecule: IL4
- Endotoxin: < 5 EU per mg of the protein.
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4.
- Molecular Mass: The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15.1 kDa as predicted.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: P05112-1
- Predicted N Terminal: Met