DESCRIPTION

• Tag: Tag Free
• Accn: NM_182691
• Type: Human Untagged Clone
• Refseq: NM_182691.1, NP_872633.1
• Status: In Stock*
• Symbol: SRPK2
• Vector: pCMV6-XL5
• Category: cDNA Clones
• Locus Id: 6733
• Synonyms: SFRSK2
• Refseq Orf: 2067 bp
• Uniprot Id: P78362
• Insert Size: 2800 bp
• Refseq Size: 3737 bp
• Availability: In Stock
• Cytogenetics: 7q22.3
• Gene Summary: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate first exon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).
• Sequence Data: Fully Sequenced ORF ( show hide ) >OriGene ORF within SC323336 sequence for NM_182691 edited (data generated by NextGen Sequencing) ATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCT CCTTTAGTTCCTCCTCCTCCACCGCCACCACCACCACCACCGCCACCTTTGCCAGACCCC ACACCCCCGGAGCCAGAGGAGGAGATCCTGGGATCAGATGATGAGGAGCAAGAGGACCCT GCGGACTACTGCAAAGGTGGATATCATCCAGTGAAAATTGGAGACCTCTTCAATGGCCGG TATCATGTTATTAGAAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTGTGCTGGGAT ATGCAGGGGAAAAGATTTGTTGCAATGATGGTTGTAAAAAGTGCCCAGCATTATACGGAG ACAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCA AACAAAGACATGGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGGGATA CATGTCTGCATGGTCTTCGAAGTACTTGGCCACCATCTCCTCAAGTGGATCATCAAATCC AACTATCAAGGCCTCCCAGTACGTTGTGTGAAGAGTATCATTCGACAGGTCCTTCAAGGG TTAGATTACTTACACAGTAAGTGCAAGATCATTCATACTGACATAAAGCCGGAAAATATC TTGATGTGTGTGGATGATGCATATGTGAGAAGAATGGCAGCTGAGGCCACTGAGTGGCAG AAAGCAGGTGCTCCTCCTCCTTCAGGGTCTGCAGTGAGTACGGCTCCACAGCAGAAACCT ATAGGAAAAATATCTAAAAACAAAAAGAAAAAACTGAAAAAGAAACAGAAGAGGCAGGCT GAGTTATTGGAGAAGCGCCTGCAGGAGATAGAAGAATTGGAGCGAGAAGCTGAAAGGAAA ATAATAGAAGAAAACATCACCTCAGCTGCACCTTCCAATGACCAGGATGGCGAATACTGC CCAGAGGTGAAACTAAAAACAACAGGATTAGAGGAGGCGGCTGAGGCAGAGACTGCAAAG GACAATGGTGAAGCTGAGGACCAGGAAGAGAAAGAAGATGCTGAGAAAGAAAACATTGAA AAAGATGAAGATGATGTAGATCAGGAACTTGCGAACGTAGACCCTACGTGGATAGAATCA CCTAAAACCAATGGCCATATTGAGAATGGCCCATTCTCACTGGAGCAGCAACTGGACGAT GAAGATGATGATGAAGAAGACTGCCCAAATCCTGAGGAATATAATCTTGATGAGCCAAAT GCAGAAAGTGATTACACATATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAAT GGACGACATAAAATTCCCGAGTCACAGTTCCCAGAGTTTTCCACCTCGTTGTTCTCTGGA TCCTTAGAACCTGTGGCCTGCGGCTCTGTGCTTTCTGAGGGATCACCACTTACTGAGCAA GAGGAGAGCAGTCCATCCCATGACAGAAGCAGAACGGTTTCAGCCTCCAGTACTGGGGAT TTGCCAAAAGCAAAAACCCGGGCAGCTGACTTGTTGGTGAATCCCCTGGATCCGCGGAAT GCAGATAAAATTAGAGTAAAAATTGCTGACCTGGGAAATGCTTGTTGGGTGCATAAACAC TTCACGGAAGACATCCAGACGCGTCAGTACCGCTCCATAGAGGTTTTAATAGGAGCGGGG TACAGCACCCCTGCGGACATCTGGAGCACGGCGTGTATGGCATTTGAGCTGGCAACGGGA GATTATTTGTTTGAACCACATTCTGGGGAAGACTATTCCAGAGACGAAGACCACATAGCC CACATCATAGAGCTGCTAGGCAGTATTCCAAGGCACTTTGCTCTATCTGGAAAATATTCT CGGGAATTCTTCAATCGCAGAGGAGAACTGCGACACATCACCAAGCTGAAGCCCTGGAGC CTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATGAAGATGCTGCACAGTTTACA GATTTCCTGATCCCGATGTTAGAAATGGTTCCAGAAAAACGAGCCTCAGCTGGCGAATGC CTTCGGCATCCTTGGTTGAATTCTTAG Clone variation with respect to NM_182691.1 329 a=>t;330 a=>g;1117 a=>g 5' Read Nucleotide Sequence ( show hide ) >OriGene 5' read for mutant NM_182691 unedited ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGAGCGAGTGGA GGCTGCAGCCCAGCTCGTCTCGGCGCCCGCGTCGCCGTCGCGAAGCCCCCCGCCCCGCTTCCGCCGCGTC GGAATGAGCTCCCGGAAAGTGCTGGCCATTCAGGCCCGAAAGCGGAGGCCGAAAAGAGAGAAACATCCGA AAAAAAATCAAGCAGAAAGATTGAGCTGCTGATGTCAGTTTAACTCTGAAAAGTCGTCCCTCTTTCAGAA AAGGCCGGGAGCCCTCAAACAGAAAAGCCTCCTTTAGTTTCCTCCTCTTCAACGGGCCAACAACCAACAA CAACCGGCAACTTTTGCCCAGAACCCCACAACCCCCCGGGACCCAAAGAAGGGAAATCCGGGGTTCGGAT AATAAGGACCAAAAGACCCCCGGCGGCCAATGCAAAGGGGGGAATTACTCAATTGAAAATTGGAGACTCC TTAATGGGCGGGAATACAGGTTAAAGAAAACTTGGATGGGGGGGACTTTTCTCCTCTGTCTGCTGTGCCT GGGATGCCCGGGGAAAGAATTGGTGCGCTGTAGGTGTGTAAAATGCGCACAATATATAGAGACACCCCTG GAGAGAATAAAATGCGCACATGTGTGTCGAAGTGATACCCGTGACCCACACAGACATGTGGTGCCACGTC ATGTCGACTCTAGATTCAGCACGATGGGAACTGTCTGCTCGGGCTCGAGATATGGCCACTCTCAGTGTTA CATCACATCAAGGCTCCGATCTGTTGAAGAGTCTCGCAGCTCTAGGTATCTACATAGTGCGAATCTCATC GTCACGGAATCTGAGTGGAG Kinase domain sequence ( show hide ) >SC323336 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation GCGCRACTCTTCATGGCCGGTATCATGTTATTAGAAGCTTGGATGGGGGCACTTCTCTACTGTCTGGCTG TGCTGGGATATGCAGGGGAAAAGATTTGTTGCAATGATGGTTGTAAAAAGTGCCCAGCATTATACGGAGA CAGCCTTGGATGAAATAAAATTGCTCAAATGTGTTCGAGAAAGTGATCCCAGTGACCCAAACAAAGACAT GGTGGTCCAGCTCATTGACGACTTCAAGATTTCAGGCATGAATGG
• Oti Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Ampicillin (100 ug/mL)
• Protein Families: Druggable Genome, Protein Kinase
• Restriction Sites: Please inquire     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: None