DESCRIPTION

• Aa: 544-976(end)
• Mw: 53.4 kDa
• Tags: N-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_000222
• Species: Human
• Uniprot: P10721
• Shiptemp: -80°C (dry ice)
• Synonyms: KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Receptor Tyrosine
• References: 1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114. 2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.
• Description: HumancKit (D816V) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of aspartic acid 816 to valine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Tyr Peptide 6, Invitrogen) at 30°C for 1 hour.
• Related Products: 40251, 40253, 40250, 79889, 78487
• Specific Activity: 397 pmol/min/µg. 
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Receptor Tyrosine