DESCRIPTION

• Aa: 1-461 (end)
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_000312
• Species: Human
• Uniprot: P04070
• Shiptemp: -80°C (dry ice)
• Synonyms: PROC, APC, activated protein C, active coagulation factor XIV
• Warnings: Avoid freeze/thaw cycles.
• Category: Protease/Protein
• References: 1. Wagener, G., et al., Transplant. Proc. 2012,44(5):1336-1340.2. Luo, L., et al., Mediators Inflamm. 2012,2012:485279.
• Description: Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 - 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.
• Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 250 mM imidazole
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl2, 0.01% Brij-35, 100 µM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460.
• Specific Activity: ≥ 95 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Protease