DESCRIPTION

• Aa: 27-282(end)
• Mw: 30 kDa + glycans
• Tags: C-terminal Avi-His Tag
• Format: Aqueous buffer solution 
• Purity: ≥90%
• Genbank: NM_001553
• Species: Human
• Uniprot: Q16270
• Shiptemp: -80°C (dry ice)
• Synonyms: Insulin-like growth factor-binding protein 7, IBP-7, IGF-binding protein 7, IGFBP-7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, Prostacyclin-stimulating factor, Tumor-derived adhesion factor (TAF), MAC25, PSF
• Warnings: Avoid freeze/thaw cycles.
• Category: Cytokine/Protein
• Description: Recombinant human CD93, also known as complement component C1q receptor, encompassing amino acids 24-580. The C-terminus is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.
• Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol 
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: human
• Product Type: Protein
• Host Cell Line: HEK293
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: IGFBP7 protein (BPS Bioscience #101545) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated CD93 protein (BPS Bioscience #101544), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
• Related Products: 101546, 101547, 101548
• Storage Stability: At least 6 months at -80°C. 
• Scientific Category: Cytokine