Anti-Sortilin (extracellular) Antibody

DESCRIPTION:

Anti-Sortilin (extracellular) Antibody (#ANT-009) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples.

DETAILS

Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Host: Rabbit
Clone: NA
Label: Unconjugated
Purity: Affinity purified on immobilized antigen.
Target: SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
Comment: Contact Alomone Labs for technical support and product customization
Gene Id: 6272
Isotype: Rabbit IgG
Homology: Mouse, rat - identical
Is Toxin: No
Sequence: DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin
Synonyms: SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
Accession: Q99523
Clonality: Polyclonal
Lead Time: 1-2 Business Days
Reactivity: Human|Rat|Mouse
Formulation: PBS pH7.4
Applications: IF|IFC|IHC|LCI|WB
Ko Validated: yes
Preservative: No Preservative
Reconstitution: 0.2 ml double distilled water (DDW).
Blocking Peptide: BLP-NT009
Negative Control: BLP-NT009
Positive Control: NA
Cited Application: IP|IHC
Immunogen Location: Extracellular domain
Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
Shipping And Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Immunogen Source Species: Human
Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
Applications May Also Work In: IF|IFC|IHC|LCI|WB
Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Product Page Scientific Background: Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has lately been identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in the brain, spinal cord, skeletal muscle and adipocytes.Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90% of sortilin is retained in intracellular compartments with only about 10% reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.
Standard Quality Control Of Each Lot: Western blot analysis
Application Dilutions Western Blot Wb: 1:400
Antibody Concentration After Reconstitution: 1 mg/ml
Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: Contact Alomone for information