DESCRIPTION

• Gene Id: 8784
• Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
• Uniprot: Q9Y5U5
• Category: ELISA
• Lead Time: 1-3 days(please inquire for mutiple units)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
• Assay Time: 3h
• Assay Type: Sandwich
• Shelf-life: one year
• Alias Names: TNFRSF18; AITR; Tumor Necrosis Factor Receptor Superfamily 18; Glucocorticoid-induced TNFR-related protein; Activation-inducible TNFR family receptor
• Sample Size: 100uL
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates and other biological fluids
• Sensitivity: 15.6-1,000pg/mL
• Specificity: Reactive with Human GITR / Glucocorticoid Induced Tumor Necrosis Factor Receptor
• Subcategory: Regular detection
• Target Name: GITR
• Research Area: Signal transduction
• Result Output: Read 450nm at Microplate reader
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
• Detection Range: 6.0pg/mL
• Detection Method: Colormetric
• Target Full Name: Glucocorticoid Induced Tumor Necrosis Factor Receptor
• Species Reactivity: Human
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37℃; 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37℃; 4. Aspirate and wash 3 times; 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37℃; 6. Aspirate and wash 5 times; 7. Add 90uL Substrate Solution. Incubate 10-20 minutes at 37℃; 8. Add 50uL Stop Solution. Read at 450nm immediately.