DESCRIPTION

• Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Kinase-Glo MAX (Promega, #V6071) Dithiothreitol (DTT, 1 M; optional) Microplate reader capable of reading luminescence Adjustable micropipettor and sterile tips30°C incubator
• Shiptemp: -80°C (dry ice)
• Synonyms: FAK, focal adhesion kinase, PTK2
• Category: Kinase/Assay Kit
• Background: FAK (Focal Adhesion Kinase; PTK2) is a protein kinase involving cell adhesion and migration. FAK plays an important role in many cancers including pancreatic tumors. In addition, recent studies showed FAK inhibitor enables an anti-tumor immune response, resulting in tumor destruction in a mouse model.
• References: Jiang, H. et al., Nature Medicine 22:851-860 (2016)
• Description: The FAK Kinase Assay Kit is designed to measure FAK kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
• Methodology: Chemiluminescent
• Supplied As: FAK Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant FAK enzyme, FAK substrate, ATP and kinase assay buffer for 100 enzyme reactions.
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 40420, 40322, 40323, 40324, 78487
• Storage Stability: Up to 6 months when stored as recommended.
• Scientific Category: Kinase
• Instructions for Use: See assay kit datasheet for detailed protocol.