Human G-CSF / CSF3 Protein (Fc Tag)

DESCRIPTION

A DNA sequence encoding the mature form of human GCSF isoform b (NP_757373.1) (Ala 30-Pro 204) was fused with the Fc region of human IgG1 at the N-terminus.

DETAILS

Tag: N-human IgG1-Fc
Purity: > 97 % as determined by SDS-PAGE
Source: HEK293 Cells
Species: Human
Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Activity: Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 0.2-0.8 ng/ml.
Molecule: CSF3
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Lead Time: 1-3 days
Formulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Molecular Mass: The recombinant mature human GCSFb/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 412 amino acids and has a calculated molecular mass of 45.4 kDa. As a result of glycosylation, the rh GCSFb/Fc monomer migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.
Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Accession Number: NP_757373.1
Predicted N Terminal: Glu 20