50121
JARID1B (KDM5B, PLU-1), His-FLAG-tags Recombinant
BPS Bioscience
DESCRIPTION
Human JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_006618), a.a. 2-751 with N-terminal His-FLAG-tag, MW=88 kDa, expressed in Sf9 cells via a baculovirus expression system.
DETAILS
- Aa: 2-751
- Mw: 88 kDa
- Tags: N-terminal His-FLAG-tags
- Format: Aqueous buffer solution
- Genbank: NM_006618
- Species: Human
- Uniprot: Q9UGL1
- Shiptemp: -80°C (dry ice)
- Synonyms: JARID1B, PLU-1, KDM5B, Retinoblastoma-Binding Protein 2 Homolog 1, RBP2-H1, CT31, Cancer/Testis Antigen 31, PLU-1, Jumonji, AT Rich Interactive Domain 1B, Lysine-Specific Demethylase 5B
- Warnings: Avoid freeze/thaw cycles.
- Category: Demethylase/Enzymes
- Background: Histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9 or H3 Lys-27. Demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
- References: 1. Yamane K, et al. (2007). Mol. Cell 25(6): 801-12.2. Barrett A, et al. (2007). Int. J. Cancer 121(2): 265-75.
- Description: Human JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_006618), a.a. 2-751 with N-terminal His-FLAG-tag, MW=88 kDa, expressed in Sf9 cells via a baculovirus expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 µg/mL FLAG peptide and 20% glycerol.
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1B (20 - 100 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
- Specific Activity: 0.22 pmole/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Demethylase