DESCRIPTION

• Notes: MATERIALS REQUIRED BUT NOT SUPPLIED: Microplate reader capable of measuring Fluorescence
• Shiptemp: -80°C (dry ice)
• Synonyms: tryptophan 5-hyrdoxylase, TRPH
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Assay Kit
• Background: Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.
• References: 1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266. 2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.
• Description: The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple -_x0001_ just mix two solutions, incubate, add quenching solution, and measure the fluorescence.
• Methodology: Fluorogenic
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 71193, 71192, 72053, 72054, 72056
• Storage Stability: At least 6 months from date of receipt when stored as directed.
• Scientific Category: Metabolic Enzyme, Immunotherapy
• Instructions for Use: See product datasheet for detailed protocol.