DESCRIPTION

• Aa: 1-302(end)
• Mw: 63 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_133494
• Species: Human
• Uniprot: Q8TDX7
• Shiptemp: -80°C (dry ice)
• Synonyms: NEK7, NIMA-related kinase 7, Never In Mitosis-Related Kinase 7
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase-Serine/Threonine/Protein
• References: 1. Kandli, M., et al. Genomics 68: 187-196 (2000). 2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).
• Description: Human NEK7, also known as NIMA Related Kinase 7 (GenBank Accession No. NM_133494), full length, a.a. 1-302 (end), with N-terminal GST-tag, MW=61.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was performed in a 25 µl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 100 µM ATP, 0.2 mg/ml Casein and NEK7 for 50 min at 30°C. NEK7 activity was measured using ADP-Glo reagents (Promega).
• Related Products: 40139, 40140, 100476, 100477, 78487
• Specific Activity: 47 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase-Serine/Threonine