E6517Hu
Human Cholesterol Side-chain Cleavage Enzyme, CYP11A1 ELISA Kit
Bioassay Technology Laboratory
DESCRIPTION
A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783).
Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783).
DETAILS
- GeneID: 1583
- Species: Human
- Storage: 2-8ºC
- MASS(Da): 60,102
- Synonyms: Cholesterol desmolase;Cholesterol side-chain cleavage enzyme, mitochondrial;CYP11A;CYP11A1;CYPXIA1;Cytochrome P450 11A1;Cytochrome P450(scc;P450SCC
- Assay Time: 1h 30m
- Assay Type: Sandwich
- Gene Names: CYP11A1
- Reactivity: Human
- Sample Type: Serum, plasma, cell culture supernates
- Sensitivity: 8.07ng/L
- Target Protein: CYP11A1
- Detection Range: 15-3000ng/L
- Full Product Name: Cholesterol side-chain cleavage enzyme
- Standard Solution: 3200ng/L
- UniProt Accession: P05108