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LIF-M5219-20ug LIF-M5219-50ug LIF-M5219-1mg

Mouse LIF Protein, Tag Free (MALS verified)

ACROBiosystems

DETAILS

  • Tag: Native
  • Host: HEK293
  • Size: 20ug
  • Buffer: PBS, pH7.4
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: Cytokines&Growth Factors
  • Molecule: LIF
  • Accession: NP_032527.1
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
  • Exp Region: Ser 24 - Phe 203
  • Swiss-prot: P09056-1
  • Characteristics: This protein carries no "tag". The protein has a calculated MW of 20.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 0.1 EU per μg
  • Molecular Weight: 20.0 kDa
  • Shipping Condition: RT
  • Species Reactivity: Mouse
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html

DESCRIPTION

Mouse LIF Protein, Tag Free (LIF-M5219) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).