50086-M27H-B
Mouse CD64 / FCGR1 Protein (His & AVI Tag), Biotinylated
Sino Biological
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DESCRIPTION
A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
DETAILS
- Tag: C-AVI & His
- Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: Mouse
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1.Immobilized Rituximab (IgG1) at 1 μg/mL can bind Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 50086-M27H-B), the EC50 is 20-50 ng/mL. 2.Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 50086-M27H-B) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.66 µM as determined in a BLI assay (Sartorius Octet Red384). 3.Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) (Cat. 50086-M27H-B) on SA Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 1.9 µM as determined in a BLI assay (Sartorius Octet Red384). 4.The purity of Mouse CD64 / FCGR1 Protein (His & AVI Tag), Biotinylated (Cat.50086-M27H-B) is more than 95% and the molecular weight of this protein is around 41-51 kDa verified by SEC-MALS.
- Molecule: FCGR1
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4
- Molecular Mass: The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 50-55 kDa due to glycosylation.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_034316.1
- Predicted N Terminal: Glu 25