TriMethyl-Histone H4-K20 Rabbit pAb

DESCRIPTION

Histone H4 ia a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

DETAILS

Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Isotype: IgG
Storage: Store at -20℃. Avoid freeze / thaw cycles.
Immunogen: A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4.
Reactivity: Human, Mouse, Rat
References: 1. Sierra F, et al. (1983)Nucleic Acids Res 11, 7069-7086.2. Marzluff W, et al. (2002) Genomics 80, 487-498.3. Lee DY, et al. (2005) Endocr Rev 26, 147-170.
Swiss Prot: P62805
Detected Mw: 11kDa
Applications: WB (1:500 - 1:2,000) IHC (1:50 - 1:200) IF (1:50 - 1:200) CHIP (1:20 - 1:100) IP (not tested) Note: Antibody dilution should be optimized by users.
Availability: In stock
Host Species: Rabbit
Purification: Protein A or G affinity purification
Also Known As: H4FA;Histone H4;HIST1H4A;HIST4H4;H4/p
Calculated Mw: 11kDa
Concentration: See labels on tube
Shipping Method: Wet ice shipping