DESCRIPTION

• Tag: N-His
• Purity: > 90 % as determined by SDS-PAGE
• Source: HEK293 Cells
• Species: Human
• Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Activity: 1.Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells. The ED50 for this effect is typically 0.2-1μg/mL. 2.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human SOST/Sclerostin Protein, His Tag (Cat. No. 10593-H07H) with an affinity constant of 39.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
• Molecule: SOST
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Lead Time: 1-3 days
• Formulation: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human SOST is a monomeric protein consists of 197 amino acids and predicts a molecular mass of 22.5 kDa. As a result of glycosylation, the apparent molecular mass of rhSOST is approximately 29.9 kDa in SDS-PAGE under reducing conditions..
• Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
• Accession Number: NP_079513.1
• Predicted N Terminal: His