Anti-ATM (C-terminal region) Antibody

DETAILS

Form: Protein A Purified
Gene: ATM
Host: Mouse
Rrid: AB_1944426
Type: Primary Antibody
Clone: M361
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: ATM (C-terminal region)
Isotype: IgG2b
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: ataxia telangiectasia mutated, AT1 ATDC TEL1 TELO1
Clonality: Mouse Monoclonal
Immunogen: Clone M361 was generated from a recombinant sequence corresponding to amino acids in the C-terminal region of human ATM.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 370 kDa* protein corresponding to the molecular mass of ATM on SDS-PAGE immunoblots of human A431 and Jurkat cells.
Applications: WB, IP, ICC, FC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 370
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Human
Target Description: Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. Activation of ATM after DNA damage involves Cdk5 mediated phosphorylation of Ser-794 followed by autophosphorylation at Ser-1891. Active ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. The Cdk5–ATM pathway regulates phosphorylation and function of the ATM targets p53 and H2AX in postmitotic neurons. Other known substrates of ATM include Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1. Thus, activation of Cdk5 by DNA damage may be an important initiator of ATM-dependent regulation of cell cycle checkpoints.
Uniprot Number Immunogen Species: Q13315