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80112-R08H

Rat Ephrin-B3 / EFNB3 Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the rat EFNB3 (G3V7D4) (Met1-Ser224) was expressed, fused with a polyhistidine tag at the C-terminus.

DETAILS

  • Tag: C-His
  • Purity: > 85 % as determined by SDS-PAGE
  • Source: HEK293 Cells
  • Species: Rat
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by its binding ability in a functional ELISA. Immobilized rat EFNB3-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His (Cat:50581-M08H), The EC50 of biotinylated mouse EphB3-His (Cat:50581-M08H) is 17.4-40.6 ng/ml.
  • Molecule: EFNB3
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4
  • Molecular Mass: The recombinant rat EFNB3 comprises 208 amino acids and predicts a molecular mass of 23.1 kDa. The apparent molecular mass of the recombinant protein is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: G3V7D4
  • Predicted N Terminal: Leu 28