40589-V08H4
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SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (HPLC-verified)
Sino Biological
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DESCRIPTION
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
DETAILS
- Tag: C-His
- Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: SARS-CoV-2
- Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Measured by its binding ability in a functional ELISA. Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4) is 10-50 ng/mL.
- Category: Proteins
- Molecule: Spike
- Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
- Lead Time: 1-3 days
- Formulation: Lyophilized from a sodium citrate buffer system at pH 6.0.
- Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P), His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: YP_009724390.1
- Predicted N Terminal: Val 16