Monoclonal Antibody to CK18 (Clone: DC10)

DESCRIPTION

Keratins, also called cytokeratins, comprise a family of filamentous structural proteins that form the intermediate filaments of epithelial cells. Keratin proteins are differentially expressed depending on epithelial cell type and degree of differentiation. Antibody to a given keratin can be useful as a marker in of itself or as part of an antibody panel to help identify or classify tissue origin.

DETAILS

Gene: KRT18
App Id: FACS, IF, WB, IHC-P
Antigen: CK18
Gene Id: 3875
Isotype: Mouse IgG1, kappa
Format Id: Purified
Clone Name: DC10
Uniprot Id: P05783
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Protein G Chromatography
Reactivity Id: Human
Product Content: 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: KRT18||CYK18||PIG46
Application Note: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Human breast cancer PMC 42 cells were used as immunogen to generate the Keratin 18 antibody.