DESCRIPTION

• Tag: Tag Free
• Accn: NM_001110149
• Type: Mouse Untagged Clone
• Refseq: NM_001110149.1, NP_001103619.1
• Status: 3 Weeks*
• Symbol: Mgat1
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 17308
• Synonyms: Mgat-1
• Refseq Orf: 1344 bp
• Uniprot Id: P27808
• Insert Size: 1344 bp
• Refseq Size: 2521 bp
• Availability: 3 Weeks
• Cytogenetics: 11 29.27 cM
• Gene Summary: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All variants encode the same isoform.
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC215949 representing NM_001110149 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGCTGAAGAAGCAGACTGCAGGGCTTGTGCTTTGGGGTGCTATCATCTTTGTGGGCTGGAATGCCCTGC TGCTTCTCTTCTTCTGGACACGCCCAGCACCTGGCAGGCTGCCCTCAGACAGCGCCCTTGGTGATGACCC TGCCAGCCTCACCCGTGAGGTCATCCACCTGGCCGAGGACGCTGAGGCGGAGTTGGAGCGGCAGAGGGGA CTGTTGCAGCAAATCAAGGAGCATTATGCTTTGTGGAGGCAGAGGTGGAGAGTTCCCACTGTGGCCCCTC CAGCCTGGCCCCGTGTGCCTGTGACCCCCTCACCAGTGCAGATCCCCATCCTGGTCATTGCCTGTGACCG CAGCACTGTCCGGCGCTGCTTGGATAAGTTGTTGCACTATCGGCCCTCAGCTGAGCGTTTCCCCATTATT GTCAGTCAGGACTGTGGGCATGAAGAGACAGCACAGGTCATTGCTTCCTATGGCACTGCAGTCACACACA TCCGGCAGCCGGACCTGAGTAACATTGCCGTGCAGCCAGACCACCGCAAGTTCCAGGGTTACTACAAGAT TGCCAGGCACTACCGCTGGGCACTAGGCCAGATCTTCAACAAGTTCAAGTTCCCGGCCGCTGTGGTAGTG GAGGATGATCTGGAAGTGGCACCAGACTTCTTTGAGTACTTCCAGGCCACCTACCCACTGCTGAGAACAG ACCCCTCCCTTTGGTGTGTGTCTGCTTGGAATGACAATGGCAAGGAGCAGATGGTAGACTCAAGCAAACC TGAGCTGCTCTATCGAACAGACTTTTTTCCTGGCCTTGGATGGCTGCTGTTGGCTGATCTGTGGGCAGAG CTGGAGCCCAAGTGGCCCAAGGCCTTTTGGGACGACTGGATGCGCCGACCTGAGCAGCGGAAAGGACGGG CTTGTATTCGTCCAGAAATTTCAAGAACTATGACCTTTGGTCGCAAGGGTGTGAGCCACGGGCAGTTCTT TGACCAGCATCTTAAGTTCATCAAGCTGAACCAGCAGTTCGTCCCCTTCACCCAGTTGGACCTGTCGTAC CTGCAGCAGGAGGCCTATGACCGGGACTTCCTCGCCCAGGTCTATGGTGCCCCCCAGCTACAGGTGGAGA AAGTAAGGACCAATGATCAGAAGGAGCTGGGGGAGGTGCGGGTACAGTACACTAGCAGAGACAGCTTCAA GGCCTTTGCTAAGGCCCTGGGTGTCATGGATGACCTCAAGTCTGGTGTCCCCAGGGCTGGCTACCGTGGC ATTGTCACTTTCCAGTTTCGGGGCCGGCGTGTGCACCTGGCACCCCCACAGACATGGACTGGCTATGATC CTAGCTGGAATTAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin