Anti-Atrogin-1 Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: Fbxo32
Host: Rat
Rrid: AB_3068356
Type: Primary Antibody
Clone: M314
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Atrogin-1
Isotype: N.T.
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: MAFbx, FBX32, Atrogin
Clonality: Rat Monoclonal
Immunogen: Clone M314 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a full length human recombinant Atrogin 1 on SDS-PAGE immunoblots.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 41
Dilution Range Wb: 1:500
Immunogen Species: Mouse
Species Reactivity: Human, Mouse, and Rat
Target Description: Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.
Uniprot Number Immunogen Species: Q9CPU7