RG239601
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CEP97 (NM_001303401) Human Tagged ORF Clone
Origene Technologies, Inc.
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DESCRIPTION:
CEP97 (tGFP-tagged) - Human centrosomal protein 97kDa (CEP97), transcript variant 2
DETAILS
- Mw: 90.8 kDa
- Tag: TurboGFP
- Accn: NM_001303401
- Refseq: NM_001303401.2
- Status: 2 Weeks*
- Symbol: CEP97
- Vector: pCMV6-AC-GFP
- Category: cDNA Clones
- Locus Id: 79598
- Orf Size: 2418 bp
- Synonyms: 2810403B08Rik; LRRIQ2
- Refseq Orf: 2421 bp
- Uniprot Id: Q8IW35
- Refseq Size: 7516 bp
- Availability: 2 Weeks
- Cytogenetics: 3q12.3
- Gene Summary: Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.[UniProtKB/Swiss-Prot Function]
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RG239601 representing NM_001303401. Blue =ORF Red =Cloning site Green =Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGC C ATGGCGGTGGCGCGCGTGGACGCGGCTTTGCCTCCCGGAGAAGGATCAGTGGTCAATTGGTCAGGACAG GGACTACAGAAATTAGGTCCAAATTTACCCTGTGAAGCTGATATTCACACTTTGATTCTGGATAAAAAT CAGATTATTAAATTGGAAAATCTGGAGAAATGCAAACGATTAATACAGTTATCAGTAGCTAATAATCGG CTGGTTCGGATGATGGGTGTGGCCAAGCTGACGTTGCTTCGTGTATTAAATTTGCCTCATAATAGCATT GGCTGTGTGGAAGGGCTAAAGGAACTAGTACATCTGGAATGGCTGAATTTGGCAGGAAATAATCTTAAG GCCATGGAACAGATCAATAGCTGCACAGCTCTACAGCATCTCGATTTATCAGACAATAATATATCCCAG ATAGGTGATCTATCTAAATTGGTATCCCTGAAAACCCTGCTTTTACATGGAAACATCATCACCTCTCTT AGAATGGCACCTGCTTACCTACCCAGAAGTCTTGCTATACTTTCTTTGGCAGAAAATGAAATCCGAGAC TTAAATGAGATCTCTTTTTTGGCATCCTTAACTGAATTGGAACAGTTGTCGATTATGAACAATCCTTGT GTGATGGCAACACCATCCATCCCAGGATTTGACTATCGGCCGTACATCGTCAGCTGGTGCCTAAACCTC AGAGTCCTAGATGGATATGTGATTTCTCAGAAGGAAAGTTTGAAAGCTGAATGGCTCTATAGTCAAGGC AAGGGGAGAGCATATCGGCCTGGCCAGCACATCCAGCTTGTCCAATATCTGGCTACAGTCTGCCCCCTC ACTTCTACACTAGGTCTTCAAACTGCAGAGGATGCCAAACTAGAGAAGATTTTGAGCAAACAGAGGTTT CACCAGAGGCAGTTGATGAACCAAAGCCAAAATGAAGAGTTGTCTCCTCTTGTTCCTGTTGAAACAAGG GCATCCCTTATTCCTGAGCATTCAAGCCCTGTTCAAGATTGCCAGATATCCCAGGAAAGTGAGTCTACT TTTATGCCAGTTGCATCAGGACTGTCTCCACTATCACCTACAGTTGAGCTGAGGCTGCAGGGCATTAAC TTGGGCCTAGAAGATGATGGTGTTGCAGATGAATCTGTGAAAGGGCTGGAAAGCCAGGTGTTGGATAAG GAAGAGGAACAGCCTTTATGGGCTGCAAATGAGAATTCTGTTCAAATGATGAGAAGTGAAATCAATACA GAGGTAAATGAGAAAGCTGGACTATTACCTTGTCCTGAGCCAACAATAATCAGTGCTATCTTGAAGGAT GATAACCACAGTCTTACATTTTTTCCTGAGTCAACTGAGCAGAAACAATCAGACATAAAGAAACCAGAA AATACACAACCAGAAAATAAAGAAACCATATCTCAAGCAACTTCAGAGAAACTTCCCATGATTTTAACC CAGAGATCTGTTGCTTTGGGACAAGACAAAGTTGCCCTTCAGAAATTAAATGATGCAGCCACCAAGCTT CAGGCCTGTTGGCGGGGATTTTATGCCAGGAACTACAACCCTCAAGCCAAAGATGTGCGTTACGAAATC CGGCTACGCAGAATGCAAGAGCACATTGTCTGCTTAACTGATGAAATAAGGAGATTACGAAAAGAAAGA GATGAAGAACGTATTAAAAAATTTGTACAAGAAGAAGCTTTCAGATTCCTTTGGAACCAGGTAAGGTCT CTACAGGTTTGGCAACAGACAGTGGACCAGCGTCTAAGTTCCTGGCATACTGATGTTCCTCCTATATCA AGTACTCTTGTGCCATCGAAACATCCATTATTTACCCAAAGCCAGGAGTCCTCTTGTGATCAAAATGCT GATTGGTTTATTGCTTCTGATGTAGCTCCTCAAGAGAAATCATTACCAGAATTTCCAGACTCTGGTTTT CATTCCTCTCTAACAGAACAAGTTCATTCATTGCAGCATTCTTTGGATTTTGAGAAAAGTTCCACAGAA GGCAGTGAAAGCTCCATAATGGGGAATTCCATTGACACAGTCAGATATGGCAAAGAATCAGATTTAGGG GATGTTAGTGAAGAACATGGTGAATGGAATAAGGAAAGCTCAAATAACGAGCAGGACAATAGTCTGCTT GAACAGTATTTAACTTCAGTTCAACAGCTGGAAGATGCTGATGAGAGGACCAATTTTGATACAGAGACA AGAGATAGCAAACTTCACATTGCTTGTTTCCCAGTACAGTTAGATACATTGTCTGACGGTGCTTCTGTA GATGAGAGTCATGGCATATCTCCTCCTTTGCAAGGTGAAATTAGCCAGACACAAGAGAATTCTAAATTA AATGCAGAAGTTCAGGGGCAGCAGCCAGAATGTGATTCTACATTTCAGCTATTGCATGTTGGTGTTACT GTG ACGCGT ACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC Protein Sequence ( show hide ) >Peptide sequence encoded by RG239601 Blue =ORF Red =Cloning site Green =Tag(s) MAVARVDAALPPGEGSVVNWSGQGLQKLGPNLPCEADIHTLILDKNQIIKLENLEKCKRLIQLSVANNR LVRMMGVAKLTLLRVLNLPHNSIGCVEGLKELVHLEWLNLAGNNLKAMEQINSCTALQHLDLSDNNISQ IGDLSKLVSLKTLLLHGNIITSLRMAPAYLPRSLAILSLAENEIRDLNEISFLASLTELEQLSIMNNPC VMATPSIPGFDYRPYIVSWCLNLRVLDGYVISQKESLKAEWLYSQGKGRAYRPGQHIQLVQYLATVCPL TSTLGLQTAEDAKLEKILSKQRFHQRQLMNQSQNEELSPLVPVETRASLIPEHSSPVQDCQISQESEST FMPVASGLSPLSPTVELRLQGINLGLEDDGVADESVKGLESQVLDKEEEQPLWAANENSVQMMRSEINT EVNEKAGLLPCPEPTIISAILKDDNHSLTFFPESTEQKQSDIKKPENTQPENKETISQATSEKLPMILT QRSVALGQDKVALQKLNDAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKER DEERIKKFVQEEAFRFLWNQVRSLQVWQQTVDQRLSSWHTDVPPISSTLVPSKHPLFTQSQESSCDQNA DWFIASDVAPQEKSLPEFPDSGFHSSLTEQVHSLQHSLDFEKSSTEGSESSIMGNSIDTVRYGKESDLG DVSEEHGEWNKESSNNEQDNSLLEQYLTSVQQLEDADERTNFDTETRDSKLHIACFPVQLDTLSDGASV DESHGISPPLQGEISQTQENSKLNAEVQGQQPECDSTFQLLHVGVTV TR TRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- E Coli Selection: Ampicillin (100 ug/mL)
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin