DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Genbank: NM_002250
• Species: human
• Shiptemp: -80°C (dry ice)
• Synonyms: IKCA1, KCNN4, Intermediate conductance calcium-activated potassium channel protein 4, IK1, hKCa4, hSK4
• Warnings: Avoid freeze/thaw cycles.
• Category: Recombinant Cell Line/Ion Channel
• Background: IKCA1 is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes.
• References: 1. Ghanshani S et al. (2000). J Biol Chem. 275(47): 37137-37149. 2. Hoffman JF et al. (2003). PNAS 100 (12): 7366-7371.3. de-Allie FA et al. (1996). Br J Pharmacol. 117(3):479-487. 4. Terstappen GC et al. (2003). Neurosci Lett. 346(1-2):85-88.
• Description: Stable, recombinant HEK293 cell line expressing human IKCA1, also known as KCNN4 (Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4, GenBank Accession No. NM_002250.
• Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: • Drug compound screening for activators or inhibitors of IKCA1 • Functional assays for IKCA1 • Efficient antigen for mouse immunization
• Host Species: human
• Product Type: Cell Line
• Host Cell Line: HEK293
• Biosafety Level: BSL-2
• Storage Stability: Store in liquid nitrogen immediately upon receipt.
• Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
• Biological Activity: N-terminal FLAG-tagged human IKCA1 has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. Functional analysis of IKCA1 channel in the stable recombinant HEK293 was accomplished by using the membrane potential-sensitive fluorescence dye DiBAC4(3). When IKCA1-expressed HEK293 cells were stimulated with calcium-specific ionophore ionomycin to raise the intracellular level of Ca2+, IKCA1 channel was activated, leading to hyperpolarization and a decrease in cell fluorescence in the functional fluorescence assay with DiBAC4(3). This decrease of cell fluorescence was blocked by the IKCA1 channel inhibitor, clotrimazole (CLT).
• Scientific Category: Ion Channel
• Instructions for Use: See data sheet for detailed culturing and assay protocol.
• Required Accessories: 60187-1,79531
• Signed License Required: Yes