DESCRIPTION

• Aa: 158-end
• Mw: 104 kDa
• Tags: N-terminal GST-tag
• Notes: Important: If you intend to assess LSD1 activity in an assay that analyzes hydrogen peroxide, such as BPS Catalog #50106 and #50107, please include a request for Peroxidase Rxn Grade LSD1 with your order.
• Format: Aqueous buffer solution
• Genbank: NM_015013
• Species: Human
• Uniprot: O60341
• Shiptemp: -80°C (dry ice)
• Synonyms: KDM1A, AOF2, lysine (K)-specific demethylase 1A, amine oxidase (flavin containing) domain 2, LSD
• Warnings: Avoid freeze/thaw cycles.
• Category: Demethylase/Enzymes
• References: 1. Lan,F. et al., Nature 448, 7154, 718-722, 2007.2. Forneris,F. et al., J. Biol. Chem. 282, 28, 20070-20074, 2007. Application Reference(s): 1. A scintillation proximity assay for histone demethylases (2014) 2. Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate (2014)
• Description: Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 10 µl reaction mix containing assay buffer with 50 mM Tris HCl pH 8.8, 50 mM NaCl, 1mM DTT, 0.01% Tween-20, histone H3 peptide substrate, and LSD1 (6.7 - 40 ng) add to the wells. Incubate for 1 hr. Add antibody against unmethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
• Specific Activity: ≥0.50 pmole/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Demethylase