mouse monoclonal IgG2a; PC-PLD2 Antibody (B-3) is an IgG2a κ mouse monoclonal PC-PLD2 antibody (also designated Phospholipase D2 antibody, Choline Phosphatase 2 antibody, Phosphatidylcholine-Hydrolyzing Phospholipase D2 antibody, PLD1C antibody, PLD 2 antibody, or HPLD2 antibody) that detects the PC-PLD2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PC-PLD2 Antibody (B-3) is available as both the non-conjugated anti-PC-PLD2 antibody form, as well as multiple conjugated forms of anti-PC-PLD2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Virtually every cell uses phosphatidylcholine as a substrate to produce phosphatidic acid and choline. Phosphatidylcholine phospholipase D1 and D2 (PC-PLD1 and PC-PLD2) are phospholipid-specific phosphodiesterases that hydrolyze phosphatidylcholine. Unlike PC-PLD1, which associates with secretory granules, PC-PLD2 localizes to the plasma membrane, where it is implicated in the formation of endocytotic vesicles. Both PC-PLD1 and PC-PLD2 coordinately regulate macrophage phagocytosis. PC-PLD activity in mammalian cells is transiently stimulated upon activation by G protein-coupled and receptor tyrosine kinase cell surface receptors. For example, PC-PLD1 and PC-PLD2 participate in sphingosine 1-phosphate stimulation of ERK phosphorylation and IL-8 secretion in bronchial epithelial cells. In addition, tubulin binding to PC-PLD2 inhibits muscarinic receptor-linked PC-PLD2 activation. PC-PLD2 also enhances PKCzeta activity through direct interaction in a lipase activity-independent manner. PC-PLD1 and PC-PLD2 stimulate cell growth by repressing expression of p21 gene through p53-dependent and p53-independent pathways, respectively, which may ultimately lead to carcinogenesis.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.